Antibiotic Susceptibility of Staphylococcus aureus from Artisanal Cheeses Sold in Markets of Jaén

Authors

DOI:

https://doi.org/10.55996/dekamuagropec.v6i1.259

Keywords:

Amoxicillin, dairy products, MRSA, resistance, healthiness

Abstract

The consumption of artisanal cheeses is high in Jaén, but the hygienic and sanitary conditions during their sale and consumption are often inadequate, increasing the risk of exposure to Staphylococcus aureus (S. aureus). This study aimed to examine the prevalence and antibiotic resistance profile of S. aureus strains isolated from artisanal cheeses distributed in Jaén markets in 2024. A total of 100 samples, each weighing 25 grams, were collected from the 25 stalls available across five local markets. The techniques used for isolating and identifying S. aureus included mannitol salt agar, Gram staining, and catalase and coagulase tests. Antibiotic resistance was assessed using the Kirby-Bauer disk diffusion method, following INS guidelines, with sensitivity disks for amoxicillin, tetracycline, ciprofloxacin, levofloxacin, and norfloxacin. Of the 55 strains identified as S. aureus, 52.7% showed resistance to amoxicillin. For fluoroquinolones (ciprofloxacin, levofloxacin, and norfloxacin), sensitivity of 96.3%, 96.3%, and 90.9% were observed, respectively. Additionally, 61.8% of strains were sensitive to tetracycline. In conclusion, the samples demonstrated resistance to amoxicillin but considerable sensitivity to tetracycline and fluoroquinolones.

Downloads

Download data is not yet available.

References

Acosta Nieves, I. P., & Roenes Galé, G. J. (2019). Staphylococcus aureus procedentes de quesos costeños de Valledupar; susceptibilidad antibiótica y perfil plasmídico. Revista Médica de Risaralda, 25(1), 10. https://doi.org/10.22517/25395203.16681

Albuja Landi, A., Escobar Arrieta, S., Guevara Iñiguez, L., Andueza Leal, F., Yugcha Pilamunga, P., & Arguello Hernández, P. (2018). Resistencia antimicrobiana de staphylococcus aureus aislado en quesos frescos artesanales elaborados en zonas rurales de antimicrobial Riobamba-Ecuador. Perfiles, 2(20), 76–81. https://doi.org/10.47187/perf.v2i20.36

Bustamante, K., Lingan, L., & Montenegro, J. (2024). Staphylococcus aureus aislados de quesos comercializados en la ciudad de Bagua, Amazonas. Revista Científica Dékamu Agropec, 5(1), 53–61. https://doi.org/10.55996/dekamuagropec.v5i1.210

Casaes Nunes, R. S., Pires de Souza, C., Pereira, K. S., Del Aguila, E. M., & Flosi Paschoalin, V. M. (2016). Identification and molecular phylogeny of coagulase-negative staphylococci isolates from Minas Frescal cheese in southeastern Brazil: Superantigenic toxin production and antibiotic resistance. Journal of Dairy Science, 99(4), 2641–2653. https://doi.org/10.3168/jds.2015-9693

Castellanos-Rozo, J., Pérez Pulido, R., Grande, M. a. J., Lucas, R., & Gálvez, A. (2021). Potentially pathogenic bacteria isolated from Paipa cheese and its susceptibility profiles to antibiotics and biocides. Brazilian Journal of Microbiology, 52(3), 1535–1543. https://doi.org/10.1007/s42770-021-00522-2

Castro, R. D., Pedroso, S. H. S. P., Sandes, S. H. C., Silva, G. O., Luiz, K. C. M., Dias, R. S., Filho, R. A. T., Figueiredo, H. C. P., Santos, S. G., Nunes, A. C., & Souza, M. R. (2020). Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from the production process of Minas artisanal cheese from the region of Campo das Vertentes, Brazil. Journal of Dairy Science, 103(3), 2098–2110. https://doi.org/10.3168/jds.2019-17138

Changanaqui, I. (2010). Presencia de Staphylococcus aureus resistente a meticilina en granjas porcinas tecnificadas del departamento de Lima.

Feyissa, N., Alemu, T., Jirata Birri, D., & Dessalegn, A. (2023). Isolation, identification, and determination of antibiogram characteristics of Staphylococcus aureus in cow milk and milk products (yoghurt and cheese) in West Showa Zone, Ethiopia. International Dairy Journal, 137, 105503. https://doi.org/10.1016/j.idairyj.2022.105503

Gajewska, J., Chajęcka-Wierzchowska, W., & Zadernowska, A. (2022). Occurrence and Characteristics of Staphylococcus aureus Strains along the Production Chain of Raw Milk Cheeses in Poland. Molecules, 27(19), 6569. https://doi.org/10.3390/molecules27196569

Kadiroğlu, P., Korel, F., & Ceylan, Ç. (2019). Identification of staphylococcus aureus cheese isolates with respect to virulence properties, genetic relatedness and antibiotic resistance profiles. Food and Health, 149–159. https://doi.org/10.3153/FH19016

Mamani, E., Luján, D., & Pajuelo, G. (2013). Perfil de sensibilidad y resistencia de Staphylococcus aureus. Experiencia en el Hospital Nacional Hipólito Unanue. Anales de La Facultad de Medicina, 67(2), 120. https://doi.org/10.15381/anales.v67i2.1249

Martínez-Vasallo, A., Ribot-Enríquez, A., Riverón-Alemán, Y., Remón-Díaz, D., Alelí Martínez-García, Y., Jacsens, L., & Uyttendaele, M. (2019). Staphylococcus aureus in the production chain of artisan fresh cheese. Revista de Salud Animal, 41(1), 2224–4700. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2019000100007&lng=es&nrm=iso&tlng=en

MIDAGRI. (2024). MIDAGRI impulsa la producción y comercialización de más de 350 mil productores de queso. Ministerio de Desarrollo Agrario y Riego. https://elperuano.pe/noticia/240104-midagri-impulsa-la-produccion-y-la-comercializacion-de-mas-de-350000-productores-de-queso

Murray, P. R., Rosenthal, K. S., & Pfaller, M. A. (2021). Microbiología Médica. Microbiología Médica Murray 9° Edición, 62, 841–853.

Organización de las Naciones Unidas para la Alimentación y la Agricultura. (2020). ¡Que fluyan los beneficios! La Ganadería y El Medio Ambiente. https://www.fao.org/newsroom/story/Pour-on-the-benefits!/es

Organización Panamericana de la Salud. (2021). Patógenos multirresistentes que son prioritarios para la OMS - OPS_OMS _ Organización Panamericana de la Salud. Https://Www.Paho.Org/Es/Noticias/4-3-2021-Patogenos-Multirresistentes-Que-Son-Prioritarios-Para-Oms. https://www.paho.org/es/noticias/4-3-2021-patogenos-multirresistentes-que-son-prioritarios-para-oms

Rafiq, K., Islam, M. R., Siddiky, N. A., Samad, M. A., Chowdhury, S., Hossain, K. M. M., Rume, F. I., Hossain, M. K., Mahbub-E-Elahi, A., Ali, M. Z., Rahman, M., Amin, M. R., Masuduzzaman, M., Ahmed, S., Ara Rumi, N., & Hossain, M. T. (2022). Antimicrobial Resistance Profile of Common Foodborne Pathogens Recovered from Livestock and Poultry in Bangladesh. Antibiotics, 11(11), 1551. https://doi.org/10.3390/antibiotics11111551

Sacsaquispe Contreras, R. E., & Velásquez Pomar, J. (2002). Manual de procedimientos anual para la prubea de sensibilidad antimicribiana por el método de disco difusión. http://www.ins.gob.pe/insvirtual/images/otrpubs/pdf/manual sensibilidad 2.pdf

Schwendimann, L., Berger, T., Graber, H.-U., Meier, S., Hummerjohann, J., & Jakob, E. (2020). Effect of Scalding Temperature on Growth of Staphylococcus aureus and Formation of Staphylococcal Enterotoxin during the Production of Alpine Cheese in a Laboratory Cheesemaking Model. Journal of Food Protection, 83(10), 1822–1828. https://doi.org/10.4315/JFP-19-600

Vargas Ovares, N. V. (2015). Importancia de las enterotoxinas producidas por Staphylococcus aureus en alimentos. Diferentes métodos de detección e identificación.

Zeinhom, M., & Abed, A. (2021). Prevalence, characterization, and control of S. aureus isolated from raw milk and Egyptian soft cheese. Journal of Veterinary Medical Research, 0–0. https://doi.org/10.21608/jvmr.2021.146885

Published

2025-06-27

How to Cite

Vásquez Requejo, J. J., Quiroz Zárate, F. A., Sánchez Mego, D. A., Pesantes Corcuera, P. E., Julca Alarcón, K. A., & Rivera Salazar, C. A. (2025). Antibiotic Susceptibility of Staphylococcus aureus from Artisanal Cheeses Sold in Markets of Jaén. Revista Científica Dékamu Agropec, 6(1), 01–06. https://doi.org/10.55996/dekamuagropec.v6i1.259