Isolation and selection of phosphorus solubilizing rhizobacteria from <i>Theobroma cacao </i> L. crops
DOI:
https://doi.org/10.55996/dekamuagropec.v1i1.21Keywords:
Phosphorus solubilizing bacteria, Theobroma cacao, plant growth promoting microorganismsAbstract
Intensive agriculture promotes the excessive use of chemical fertilizers that cause the immobilization of nutrients in the soil, opting as an altemative solution the use of fertilizers based on microorganisms. This research work aimed to isolate and select native bacteria with phosphorus solubilizing capacity from samples of Theobroma cacao rhizospheric soil collected in the provinces of Bagua and Utcubamba in the Amazon region. The 23 processed soil samples presented mainly silt-free texture, anda variable pH between 6.24 and 7.75, ofwhich 42 rhizobacterial cultures from the Bagua, Vista Hermosa, Los Olivos, Santa Teresa, El Vivero, La Peca and Cajaruro sectors were isolated from the provinces of Bagua and Utcubamba. Primary isolation was performed in nonselective culture medium and secondary selection in Pikovskaya medium, with halos of solubilization of tricalcium phosphate; At the same time, macro and microscopic characterization of isolated colonies with solubilizing capacity was performed. Solubilization Indices (IS) were measured on plate of each isolated pure culture, selecting two super solubilizing cultures with IS> 3. It is concluded that from the rhizosphere of Theobroma cacao it was possible to isolate several pure cultures of phosphorus solubilizing bacteria, two ofthem being selected as super solubilizers, which were preserved in culture medium added with glycerol for later identification.
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